biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:499-504, 2005 | DOI: 10.1007/s10535-005-0041-6

Isolation of a differentially spliced C-type flower specific AG-like MADS-box gene from Crocus sativus and characterization of its expression

A. S. Tsaftaris1,2,*, K. Pasentsis1, A. N. Polidoros1
1 Institute of Agrobiotechnology, CERTH, Thermi, Greece
2 Department of Genetics and Plant Breeding, AUTH, Thessaloniki, Greece

We have cloned and characterized the expression of Crocus sativus AGAMOUS1 (CsAG1), a putative C-type MADS-box gene homologous to AGAMOUS (AG) from a triploid monocot species crocus (Crocus sativus L.). The typical domain structure of MIKC-type plant MADS proteins was identified. Phylogenetic analysis of the deduced amino acid sequence indicated that the isolated gene forms a clade with the AGAMOUS homologs from the monocots Hyacinthus orientalis and Phalaenopsis equestris. A differential splicing event altering the amino acid sequence at the C terminus was identified, leading to the formation of two mRNAs differing ten nucleotides in size. The presence of both differentially spliced transcripts was restricted only to mature crocus flowers and particularly to stamens and carpels.

Keywords: AGAMOUS; monocot; saffron; phylogeny
Subjects: amino acids; Arabidopsis thaliana; Brassica napus; Corylus avellana; Crocus sativus; dendrogram, cladogram, phenogram, phylogenetic tree; flower; Gerbera hybrida; Helianthus annuus; Hyacinthus orientalis; Lycopersicon esculentum; MADS-box gene; Magnolia precocissima; Nicotiana tabacum; Panax ginseng; Petunia hybrida; Phalaenopsis equestris; polymerase chain reaction (PCR); Rosa rugosa; saffron; Southern blot analysis; Vitis vinifera; Zea mays

Received: March 11, 2004; Accepted: April 14, 2005; Published: December 1, 2005  Show citation

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Tsaftaris, A.S., Pasentsis, K., & Polidoros, A.N. (2005). Isolation of a differentially spliced C-type flower specific AG-like MADS-box gene from Crocus sativus and characterization of its expression. Biologia plantarum, 49(4), 499-504. doi: 10.1007/s10535-005-0041-6
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